Sandwich enzyme-linked immunosorbent assays (ELISAs) are the most frequently used ELISA format. It requires the use of matched antibody pairs whereby each antibody is specific for different non-overlapping epitopes on the antigen. Firstly, one known as the capture antibody is coated onto the surface of a 96-well plate to facilitate the immobilization of the target antigen. Then the detection antibody binds to the capture antibody-antigen complex. sandwich elisa advantages https://www.antibody-creativeb....iolabs.com/sandwich-

Sandwich ELISA with Direct Detection - Creative Biolabs

Creative Biolabs summarizes the basic principles and technical points of sandwich ELISA with direct detection.